My biological mother is deceased and my auntie and other lady helped to raise me. How can I honor them both on that day.

You can have them ushered in and seated in the first row, where parents would sit. And be sure to have a corsage for both. Some brides/grooms as part of the service, walk down and present a rose to each of the mothers/grandmothers, and along with the groom's mother, you could give each of them a rose.

Seat them at the reception as though they were your parents. Some have a parents table with both sides together, some have the families separated - depending on the size of the families, etc.